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Waterloo/13 August 2008
From 2008.igem.org
08/08/13 Wed
[x] gel of full digestion (c0052', c0051', T7 gene6-27)
[x] gel extraction
[x] check gel of extraction
[x] ligation of parts from extraction and c0052', c0051'
[x] transformation (extraction and c0052', c0051')
[] p/s/i of transformants
[x] glycerol stock of S.meliloti (x3)
[x] reinoculate C0052', c0051' in Ap
[x] another PCR of PHB A and B (leave overnight)
[] send 2 of each gene for sequencing
[x] PCR of PHB genes (if primers arrive)
- try using Taq first
- only PHBC worked
[x] check gel of PCR
[x] if Taq PCR successful, run again w/ Phusion; else run troubleshooting PCR w/ Taq
