Brown: Team Resistance/8 June 2008

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Toxipop lab notebook.png

June 8, 2008

Considerations for gene cassette

Harvard’s paper: Use of Lambda Phage S and R Gene Products in an Inducible Lysis (Jain and Mekalanos) System for Vibrio cholerae- and Salmonella enterica Serovar Typhimurium-Based DNA Vaccine Delivery Systems

  • Used luria lb
  • Grew cells up to mid-logarithmic stage then added the arabinose
  • Saw decrease in cell density after just 1 hour, but no change in the control
  • Used plasmid pvj4
  • E.coli strains:

DH5alpha Beta2155

  • Can we check tightness of promoters by using gfp?

Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow)

Requested meeting with Professor Palmore in the engineering department to talk about specifications of our apparatus (metal used for electrodes, circuit design, labview programming)

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