Team:Hawaii/Notebook/2008-10-24

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Things we did today

Wetlab work

Prep for sequencing BB1

Grace
  • ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing

Plasmid prep of parts to send to iGEM

Grace
Plasmid Concentration
nir
slr
pilA
GFPf
nir+rbs
slr+GFPf
BBpRL1383a #1 from sp100 plate

Antibiotic test

Grace
Restreak of transformants
Plate Colony Growth? Blue?
LB+amp100 sp2.5 blue NoNo
sp100 #1NoNo
sp100 #2NoNo
LBalign="center"|sp2.5 blue YesYes, ~20
sp100 #1YesNo
sp100 #2YesNo
LB+sp100sp2.5 blue NoNo
sp100 #1YesNo
sp100 #2YesNo


  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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