"
Team:Montreal/DNA Agarose Gel
From 2008.igem.org
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
|---|
Procedure
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
