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Team:Chiba/Calendar-Home/23 September 2008
From 2008.igem.org
22 September 2008 <|> 24 September 2008
Laboratory work
Team:Communication
- time-delay test
- transformants
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084012 BBa_K084012]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014]
- E.coli strain
Senders:XL10GOLD
Receiver:JW1908 - reaction temparature
37°C and 30°C
- transformants
- Result(at 37°C)
- Receiver cells/Sender cells = 1
- Receiver cells/Sender cells = 10
- Result(at 30°C)
- Receiver cells/Sender cells = 1
- Receiver cells/Sender cells = 10
Team:Output
Digestion test
BBa_E0240
| DNA | 2 |
| EcoRⅠ | 0.5 |
| PstⅠ | 0.5 |
| BSA(x10) | 1 |
| EcoRⅠ Buffer(x10) | 1 |
| dH2O | 5 |
| TOTAL | 10 |
TRANSFORMATION
BBa_I6107
BBa_I6206
BBa_I6101
BBa_I6042
BBa_I0462
Digestion
Vector:BBa_R0062①
Insert:BBa_E0240②
Vector:BBa_I7100③
Insert:BBa_R0062④
Insert:BBa_J37033⑤
| Sample No | ① | ② | ③ | ④ | ⑤ |
| DNA | 25 | 25 | 25 | 25 | 25 |
| EcoRⅠ | - | - | 1 | 1 | - |
| SpeⅠ | 1 | - | 1 | 1 | - |
| PstⅠ | 1 | 1 | 1 | - | 1 |
| XbaⅠ | - | 1 | - | - | 1 | BSA(x10) | 4 | 4 | 4 | - | 4 |
| EcoRⅠ Buffer(x10) | - | - | 4 | 4 | - |
| Buffer 2 | 4 | - | - | - | - |
| Buffer 3 | - | 4 | - | - | 4 |
| dH2O | 5 | 5 | 5 | 9 | 5 |
| TOTAL | 40 | 40 | 40 | 40 | 40 |
->1hour 37℃
->SAP 1μl(Vector only) ->1hour 37℃ ->30min 65℃
->Zymo ->10μl
Gel cheack
| DNA | 1 |
| Loading Dye | 1 |
| dH2O | 4 |
| TOTAL | 6 |
->③&④&⑤のLigationはしない
