Team:Warsaw/Calendar-Main/1 September 2008

From 2008.igem.org

Revision as of 19:18, 26 October 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha

Piotr

pET15b+A_alpha in Rosetta strain inoculated in liquid LB with chloramphenicol and ampicillin.

Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
Results (% of blue colonies):
pMPMT5-omega 100%
pMPMT5-AID 100%
pMPMT5-AIDT7 50%
pMPMT5-AID+T7 0% (weird)

Results of rifampicin test
StrainInducer / repressorNumber of colonies on rifampicin
GM2163none1
GM2163 pMPM-AID0.1% Arabinose47
GM2163 pMPM-AIDnone4
GM2163 pMPM-AID+T70.1% Arabinose99
GM2163 pMPM-AID+T7none2
GM2163 pMPM-AIDT70.1% Arabinose12
GM2163 pMPM-AIDT7none9

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008"