Team:Warsaw/Calendar-Main/1 September 2008

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Purification of proteins: A-alpha

Piotr

Liquid LB with chloramphenicol and ampicillin was inoculated with Rosetta strain carrying pET15b+A_alpha.

Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
Results (% of blue colonies):
pMPMT5-omega 100%
pMPMT5-AID 100%
pMPMT5-AIDT7 50%
pMPMT5-AID+T7 0% (weird)

Results of rifampicin test
StrainInducer / repressorNumber of colonies on rifampicin
GM2163none1
GM2163 pMPM-AID0.1% Arabinose47
GM2163 pMPM-AIDnone4
GM2163 pMPM-AID+T70.1% Arabinose99
GM2163 pMPM-AID+T7none2
GM2163 pMPM-AIDT70.1% Arabinose12
GM2163 pMPM-AIDT7none9

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