USTC/Notebook/Preparing Antibiotic Stocks

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Contents

Preparing Antibiotic Stocks

Ampicillin

Stocks & Usage

  • Stock Concentration - 50mg/ml in H2O
  • Aliquots - 100μl and 500μl
  • Working Concentration - 50μg/ml

Kanamycin

Stocks & Usage

  • Stock Concentration - 10mg/ml in H2O
  • Aliquots - 200μl and 1ml
  • Working Concentration - 20μg/ml

Chloramphenicol

Stocks & Usage

  • Stock concentration - 34mg/ml in 100% Ethanol
  • Aliquots - 1ml
  • Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)