Alberta NINT/27 May 2008
From 2008.igem.org
lab work (SD):
DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected. Ligated K102000 with E0433 and K102000 with null_output.
lab work (JD):
Recovered Part BBA_R011 from iGEM package Transformed XL1-B cells and plated on LB+Amp100 plates. Created 2 O/Ns as well.