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Team:Warsaw/Calendar-Main/27 September 2008
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Revision as of 18:35, 27 October 2008 by
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Mutagenesis of protein A
Paweł
Isolation of plasmid DNA from overnight cultures (it was really hard work...).
Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
Results of digest:
ADelL+KpnI and ADelP primers mutagenesis:
pACYClac+ompA-ΔA-omega
:
0
positive,
5
negative
pACYClac+ompa-ΔA-alpha
:
3
positive,
7
negative
pACYClac+OmpA-omega-ΔA
:
1
positive,
2
negative
AMutL+KpnI and AMutP mutagenesis:
pACYClac+ompA-ΔA-omega
:
4
positive,
16
negative
pACYClac+ompa-ΔA-alpha
:
4
positive,
8
negative
pACYClac+OmpA-omega-ΔA
:
2
positive,
15
negative
Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).