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Team:NTU-Singapore/Notebook/18 July 2008
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NTU@iGEM
Project
Introduction
Methodology
Team
Introduction
Team background
Notebook
Dry Lab
Introduction
ODEs used
Modeling Constructs
Parameters used
Deterministic Modeling
Sensitivity Analysis
Stochastic Modeling
Wetlab
Introduction
Materials and Equipment
Protocols
Verification of Detection & Lysis System
Our New Parts
Characterization
Introduction
Standard Promoters
Characterization of pLacI-GFP
pLacI-GFP images and clips
Characterization of pLsrA-YFP
New characterization Proposal
Parameter Estimation & Correlations
Acknowledgements
Friday 18 July
RESULTS FROM THURSDAY TRANSFORMATION:
Plate of E7 got 4 colonies!!
Plate of T7ptag got 8 colonies!!
We also received LsrA and Lysis (lyophilized form) in Bluescript vector. We eluted them using TE buffer.
Afternoon:
1600: transform lysis and LsrA (0.3ul each) into competent cells.
1700: inoculate 4 colonies of E7 and 8 of T7ptag.