Team:Princeton/Experiments

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PRINCETON IGEM 2008

Home	Project Overview	Project Details	Experiments	Results	Notebook

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Bio-brick Creation

Lab Protocols

1. Primer Design 2. PCR 3. Run Gel 4. Gel Extraction 5. Digestion 6. CIP Treatment 7. PCR Purification 8. Ligation 9. Transformation and Plating 10. Extract DNA (Miniprep) 11. Restriction Map/ Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence

Cell Culture