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Team:Princeton/Experiments
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PRINCETON IGEM 2008
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Bio-brick Creation
Lab Protocols
1. Primer Design
2. PCR
3. Run Gel
4. Gel Extraction
5. Digestion
6. CIP Treatment
7. PCR SOEing
8. Ligation
9. Transformation and Plating
10. Extract DNA (Miniprep)
11. Restriction Map/ Digest
12. Re-transform with selected plasmid
13. Extract DNA (Maxiprep or Midiprep)
14. Sequence
Bio-Brick Verification
Cell Culture Process
1. Lentivirus Production
2. Lentivirus Harvesting
3. Infection
Also,
1. Transfection (nonviral)
