Experiment

From 2008.igem.org

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	Experiment results Synthetic convertible ecosystem EG cells received 3OC6HSL signal molecular and expressed ampicillin resistance protein   ER cells received C4HSL signal molecular and expressed kanamycin resistance protein The growth curve and the expression of the GFP of the EG cells in different condition The growth curve and the expression of the GFP of the ER cells in different condition ER overwhelms EG in population at the existence of antibiotics   Antibiotic changes detected by LC-MS/MS   Both ampicillin and kanamycin decrease with the increase of population density due to gradual decomposition of antibiotics. Most of antibiotics were decomposed at the lag phase and decreased very quickly to a low level at the beginning of the exponential phase. Increase of initial population density enhances system resistance to antibiotics EG= 3.33×106/ml ER= 1×106/ml   EG= 5×106/ml ER= 1×106/ml   EG= 1×107/ml ER= 1×106/ml   AMP=4mg/ml KAN=1mg/ml   AMP=8mg/ml KAN=1mg/ml   AMP=8mg/ml KAN=1.6mg/ml simulation   Influence of antibiotics on population dynamics EG= 1×107/ml   ER= 1×106/ml   EG= 5×106/ml ER= 1×106/ml simulation

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