Wisconsin:Lignin Project/7 August 2008

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Team Sorbitol:

Set up a Digestion of the srlD PCR product as well as the pET28a vector. The Products were verified on a gel and extracted using a gel purification kit.

Team Fungus:
Transformed into Rosetta-gami 2(DE3)pLysS.
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media.