Team:Hawaii/Protocols

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List of Protocols

This is a list of tried and true protocols used by our team.

Making competent cells
Transformation of E. coli
Triparental conjugation of Synechocystis sp. PCC6803
Alkaline lysis / plasmid isolation
Solid growth media

LB
LB + amp₁₀₀
LB + kan₂₀ + amp ₅₀
LB + sp₁₀₀
LB + sp₁₀₀ + sm₅₀
BG-11 + 5% LB
BG-11
BG-11 + sp_2.5 + sm_2.5

Liquid growth media

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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