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Team:Davidson-Missouri Western/New Parts Contributed to the Registry
From 2008.igem.org
The Davidson-Missouri Western team contributed 105 new parts to the Registry of Standard Biological Parts, including several novel hybrid promoters which have been designed to be dually-regulated, testing constructs, and selected intermediate parts. The new basic parts were sequence verified before continuing the building process to ensure against mutations.
| Number | Type | Description | Size | |
|---|---|---|---|---|
| BBa_K09100 | Composite | Receiver for AHL and Outputs GFP when AHL is present | 1946 bp | |
| BBa_K091001 | Coding | LsrR gene | 954 | |
| BBa_K091002 | Coding | LsrK gene | 1593 | |
| BBa_K091100 | Regulatory | pLac_lux hybrid promoter | 74 | |
| BBa_K091101 | Regulatory | pTet_Lac hybrid promoter | 83 | |
| BBa_K091104 | Regulatory | Mnt/LacI Hybrid Promoter | 87 | |
| BBa_K091105 | Regulatory | Mnt/TetR Hybrid Promoter | 98 | |
| BBa_K091106 | Regulatory | LsrA/cI hybrid promoter | 141 | |
| BBa_K091107 | Regulatory | Lux-cI hybrid promoter | 57 | |
| BBa_K091109 | Coding | LuxS | 516 | |
| BBa_K091110 | Regulatory | LacI Promoter | 56 | |
| BBa_K091111 | Regulatory | LacIQ promoter | 56 | |
| BBa_K091112 | Regulatory | LacIQ1 promoter | 56 | |
| BBa_K091114 | Regulatory | LsrAR Promoter | 248 | |
| BBa_K091115 | Regulatory | LsrR Promoter | 100 | |
| BBa_K091116 | Regulatory | LsrA Promoter | 126 | |
| BBa_K091117 | Regulatory | pLas promoter | 126 | |
| BBa_K091118 | Intermediate | Codes for LasR and LasR regulated GFP | 1985 | |
| BBa_K091119 | Intermediate | LacI protein generator with a pTet promoter | 74 | |
| BBa_K091120 | Intermediate | pTet regulated production of LacI protein | 1163 | |
| BBa_K091121 | Coding | LacI wild-type gene | 1083 | |
| BBa_K091122 | Coding | LacI_I12 protein | 1083 | |
| BBa_K091123 | Composite | LacI_I12 protein regulated by pTet | 1163 | |
| BBa_K091125 | Composite | GFP generator regulated by pTet | 938 | |
| BBa_K091126 | Composite | pTet regulated production of LacI_X86 | 1163 | |
| BBa_K091127 | Coding | LacI_I12_X86 | 1083 | |
| BBa_K091128 | Composite | pTet regulation of the LacI_I12_X86 protein | 1163 | |
| BBa_K091131 | Generator | pLacIQ1 controlled GFP generator | 940 | |
| BBa_K091132 | Composite | pMnt/Tet+RBS+LuxI | 767 | |
| BBa_K091133 | Composite | pBAD+RBS+TetR | 841 | |
| BBa_K091134 | Device | Las Receiver | 2048 | |
| BBa_K091136 | Signalling | Las Sender Testing Device | 670 | |
| BBa_K091137 | Coding | RBS+Mnt+TT | 468 | |
| BBa_K091138 | Composite | RBS+lsrR | 972 | |
| BBa_K091139 | Composite | RBS+lsrR+TT | 1109 | |
| BBa_K091140 | Composite | plsrA/cI+RBS+luxI | 810 | |
| BBa_K091141 | Composite | RBS+Mnt+TT | 468 | |
| BBa_K091142 | Intermediate | plsrA/cI+RBS+luxI+RBS+Mnt+TT | 1286 | |
| BBa_K091143 | Regulatory | plasR/cI Hybrid Promoter | 164 | |
| BBa_K091144 | Composite | pMnt/Tet+RBS+LuxI+RBS+cI+TT | 1671 | |
| BBa_K091145 | Composite | pBAD+RBS+TetR+TT | 978 | |
| BBa_K091146 | Regulatory | pLas+ Lux- Promoter | 126 | |
| BBa_K091147 | Composite | pBAD+RBS+Mnt+TT | 606 | |
| BBa_K091148 | Generator | pLas' Promoter Testing Construct A | 1997 | |
| BBa_K091149 | Signalling | pLas' Testing Construct B | 1997 | |
| BBa_K091150 | Signalling | pLas' Testing Construct C | 2804 | |
| BBa_K091151 | Signalling | pLas' Testing Construct D | 2761 | |
| BBa_K091152 | Signalling | pLas' Testing Construct D | 2804 | |
| BBa_K091153 | Signalling | Standard RBS with LuxS | 534 | |
| BBa_K091154 | Composite | RBS+luxI+RBS+Mnt+TT | 1137 | |
| BBa_K091155 | Intermediate | plasR/cI+RBS+luxI+RBS+Mnt+TT | 1309 | |
| BBa_K091156 | Regulatory | pLux | 55 | |
| BBa_K091157 | Regulatory | pLux+ Las- Promoter | 55 | |
| BBa_K091158 | Composite | RBS+lsrK | 1611 | |
| BBa_K091159 | Composite | pBAD+RBS+lsrK | 1749 | |
| BBa_K091160 | Composite | pMnt/Tet-RBS-GFP-TT | 982 | |
| BBa_K091161 | Signalling | RBS+LasI+RBS+Mnt+TT | 1103 | |
| BBa_K091162 | Signalling | Promoter+RBS+LasI+RBS+Mnt+TT | 1168 | |
| BBa_K091163 | Signalling | RBS+LuxS+RBS+Mnt+TT | 1010 | |
| BBa_K091164 | Signalling | Promoter+RBS+LuxS+RBS+Mnt+TT | 1075 | |
| BBa_K091165 | Reporter | pLux/cI+RBS+GFP+TT | 941 | |
| BBa_K091166 | Signalling | pBAD-RBS-luxR | 937 | |
| BBa_K091167 | Signalling | RBS+LuxI+TT | 798 | |
| BBa_K091168 | Composite | pLac/Mnt+RBS+LuxS+RBS+cI+TT | 1533 | |
| BBa_K091169 | Composite | plasR/cI+E0240(GFP) | 1048 | |
| BBa_K091170 | Composite | plsrA/cI+E0240(GFP) | 1025 | |
| BBa_K091171 | Composite | pBAD+RBS+cI+TT | 1034 | |
| BBa_K091172 | Intermediate | lsr system supporting genes | 2866 | |
| BBa_K091173 | Device | pLsr/cI+RBS+luxI+RBS+Mnt+TT+pTet/Mnt+RBS+luxI+RBS+cI+TT | 2965 | |
| BBa_K091174 | Device | pBAD-RBS-TetR-TT-pBAD-RBS-LsrK-RBS-LsrR-TT | 3852 | |
| BBa_K091175 | Composite | pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT | 1968 | |
| BBa_K091176 | Composite | pBAD-RBS-Mnt-TT-pMnt/tetR-RBS-GFP-TT | 1596 | |
| BBa_K091177 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT | 1968 | |
| BBa_K091178 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT | 1596 | |
| BBa_K091179 | Signalling | LuxR receiver and pBAD promoter | 1074 | |
| BBa_K091180 | Composite | pLac-RBS-LasR-TT | 999 | |
| BBa_K091181 | Composite | pBad-RBS-tetR-TT-placI/pI-RBS-lasR-TT | 1985 | |
| BBa_K091182 | Composite | pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT | 2988 | |
| BBa_K091183 | Signalling | pBAD+RBS+LuxR+RBS+cI lam+TT | 1841 | |
| BBa_K091184 | Regulatory | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT | 2616 | |
| BBa_K091185 | Composite | LacI_I12+TT | 1220 | |
| BBa_K091186 | Composite | pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | 2528 | |
| BBa_K091188 | Composite | pMnt/lac+RBS+lasI+RBS+cI+TT | 1626 | |
| BBa_K091190 | Composite | plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT | 2802 | |
| BBa_K091191 | Composite | pBAD+RBS+luxS+TT | 809 | |
| BBa_K091192 | Composite | pBAD+RBS+lasI+TT | 807 | |
| BBa_K091194 | Reporter | Promoter testing construct | 2023 | |
| BBa_K091195 | Reporter | Promoter testing construct | 1983 | |
| BBa_K091196 | Reporter | Promoter testing construct | 2790 | |
| BBa_K091197 | Intermediate | plsrAcI testing construct with GFP | 3899 | |
| BBa_K091198 | Intermediate | plasRcI testing construct | 2055 | |
| BBa_K091199 | Intermediate | plasR/cI overall testing construct | 3097 | |
| BBa_K091200 | Intermediate | plsrA/cI testing construct with lambda cI repression | 2067 | |
| BBa_K091201 | Intermediate | plsrAcI overall testing construct | 4941 | |
| BBa_K091202 | Intermediate | plasRcI testing construct with lambda cI repression | 2090 | |
| BBa_K091203 | Device | pBAD-RBS-LuxR-RBS-LacI(I12)-TT | 2183 | |
| BBa_K091204 | Coding | Prom-LuxR-TT | 979 | |
| BBa_K091205 | Coding | Prom-LasR-TT | 979 | |
| BBa_K091206 | Coding | Prom-LuxR-TT-Prom-LasR-TT | 1966 | |
| BBa_S03967 | Intermediate | RBS-LasI | 627 | |
| BBa_S03968 | Intermediate | RBS-LuxS | 534 | |
| BBa_S03969 | Intermediate | pMnt/Lac-RBS-GFP-TT | 971 | |
| BBa_S03979 | Intermediate | RBS-LuxS-RBS-cI-TT | 1438 | |
| BBa_S03980 | Intermediate | pBAD-RBS-LacI-TT | 1446 | |
| BBa_S04058 | Intermediate | RBS-LacI12-TT | 1238 |
