Team:The University of Alberta/21 October 2008

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i digested: 

  pUC57-pTET-BisdA with SphI+PstI with CIP treatment
  pTET-BisdB with XbaI+PstI
  laqIQ-ERE-TetR with XbaI+PstI

i set up the following ligations with the gel purified products: 

  pUC57-pTET-BisdA+pTET-BisdB
  pSB103+laqIQ-ERE-TetR

overnight at 16 degrees C.

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