Team:The University of Alberta/21 October 2008

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David

digested:

  pUC57-pTET-BisdA with SphI+PstI with CIP treatment
  pTET-BisdB with XbaI+PstI
  laqIQ-ERE-TetR with XbaI+PstI

set up the following ligations with the gel purified products:

  pUC57-pTET-BisdA+pTET-BisdB
  pSB1A3+laqIQ-ERE-TetR

overnight at 16 degrees C.

TOm

sequenced ETFA/B col5. with reverse universal

Benny

sequenced ETFA/B col5. with Vf and Vr
sequenced 21-25 col1. with Vf and Vr
digested 21-25, ETFA/B with X/P
run digest on a gel

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