Due to the nature of the Cell Lysis Cassette, Optical Densities can be used to determine when lysis has occurred. The R gene (endolysin) of the cassette degrades the cell wall. The solution becomes clear after lysis. After two to three hours, the Optical Density of the cell cultures dropped, signifying the expression of the S,R,Rz genes. The following graphs exhibit optical density trends during gene expression and resulting cell lysis and cell wall degradation.
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