Team:Heidelberg/Notebook/Killing I/Notebook/week12

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Week 12

Monday, 10/20/08

Proceedings of phage cloning strategy one

  • the fully mutated insert in pBluescript SK II was digested with XbaI/XhoI and the insert extracted from the gel
  • lambda DNA was also cut with XbaI/XhoI and the backbone also extracted from the gel.
  • the insert was ligated in the lambda backbone over night using standard ligation methods


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