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Team:Rice University/Notebook/12 June 2008
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Thursday 12 June
- Taylor Stevenson
- Transformation From New Registry (Paper Punches)-Electroporation and Heat Shock of [http://partsregistry.org/Part:BBa_I744204 BBa_I744204] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] (positive control) and [http://partsregistry.org/Part:BBa_I13521 BBa_I13521] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into XL1-Blue MR electrocompetent and XL1-Blue chemically competent cells.
- Added 1uL of stock miniprepped [http://partsregistry.org/Part:BBa_I744204 BBa_I744204] DNA solution and 2uL obtained by soaking [http://partsregistry.org/Part:BBa_I13521 BBa_I13521] DNA punch in (10:1) Tris:EDTA buffer incubated @ 50*C for 30min to both chemical and electrocompetent cells.
- Electropulse parameters for the miniprepped plasmid and extracted plasmid where 1.8kV & 5.2ms and 1.8kV & 5.0ms respectively.
- Plated 100uL of each the transformed and cultured cells on LB/Amp plates. Plates were cultured O/N @ 37*C.
- Result-no colonies were observed on plates from the extracted DNA while >>1,000 colonies grew on plates resulting from miniprepped DNA.
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