Team:Rice University/Notebook/5 June 2008

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Thursday 5 June

  • Selim Sheikh:
    • Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
20080604 gel.jpg

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)










  • Taylor Stevenson
    • Titered the phage stocks obtained from packaging the NEB lambda genome
    1. Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm.
    2. Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04. Aliquot into 200uL fractions.
    3. Add serial dilutions of phage in SM buffer see media:packaging manual.pdf
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