"
Team:Rice University/Notebook/5 June 2008
From 2008.igem.org
Revision as of 18:10, 25 June 2008 by StevensonT (Talk | contribs)
Thursday 5 June
- Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)
- Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome see packaging manual.pdf
- Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
