Team:Rice University/Notebook/14 June 2008

From 2008.igem.org

Revision as of 20:13, 25 June 2008 by StevensonT (Talk | contribs)

Saturday 14 June

  • Taylor Stevenson
    • Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
    • Result-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.

20080614 30*C VCS lysogen.jpg 20080614 42*C VCS lysogen.jpg










Home The Team The Project Parts Submitted to the Registry Modeling Notebook
Retrieved from "http://2008.igem.org/Team:Rice_University/Notebook/14_June_2008"