Team:Rice University/Notebook/14 June 2008

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Saturday 14 June

  • Taylor Stevenson
    • Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
    • Result-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.

20080614 30*C VCS lysogen.jpg 20080614 42*C VCS lysogen.jpg






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