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_meetings
Sept. 24th. 2008
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Sept. 29th 2008
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Oct. 7th 2008'
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Oct. 17th 2008
participants:
Philipp Mappes, Katja Arndt, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib
Final report
- structure equal to a scientific paper containing the parts Abstract, Introduction, Materials and Methods, Results
- fusion of all sections we worked in: Origami, AFM, FACS, CA2+/Fura measuremt, cell cultures, modeling, ethics
- quotation of literature through autor and year of publication in the text (Max Muster, 2007), full quotation in references in the end
- deadline Friday Oct. 24th 2008
official wikipediasite
- continue the labjournal on the official page only
- not too much linking: maintenance of the flow of information
- all embedded files should start with "Freiburg2008"
iGEM parts
- uploading the parts starting on Monday Oct. 20th 2008
Oct. 21th 2008
participants:
Moritz Busaker, Philipp Mappes, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib, Kathrin Pieper, Simone Weber
Cloning:
At least - All the constructes are cloned!!!
construcs:
pMA - singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - X – pMA
X :
- bla1(1/2 beta-Lactamase)-
- bla2 (1/2 beta-Lactamase) –
- split-linker-C-CFP(Cerutan) –
- N-CFP –
- split-linker-C-GFP (split venus)– --------> Hi Philipp oder Robert!!! könnt ihr von bla1 bis Luciferase2 ein Stück einziehen?! Habs nicht hin bekommen
- N-GFP –
- luciferase 1 (58) –
- luciferase 2 (57) –
Next steps:
1) We want to test, if the transmembran region really is located in the membrane
Therefore we want to clone a constructe which expresses a YFP on the cytoplasmic side of the receptor.
Construct:
- singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - split-linker - bla1 - YFP -
2) We also want to test, if we able to bringe the receptors together by using a NIP coupled peptide.
Therefore we need the peptides from Schamels group -> Norman will ask them.
3) Origami-We want to try if the Origami are able to bring two or more receptors together.
Have to produce new Origami -> Michael and Norman
Next meeting: Oct. 23th 2008
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