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Sept. 18th 2008

attendees
Philipp Mappes, Katja Arndt, Kristian Müller, Normann Kilb, Robert Gawlik, Simone Weber, Kathrin Pieper, Sabine Jägle

organisatory

• Bioss agrees to sponsorship
• team picture for wiki
• next meeting: wednesday, Sept. 24th 15.30
• lab: new box-arrangement (Normann)
• table for cloning strategy (Kathrin)

Ca-Messung

• date for measurement at the AFM (Nitschke)
• optionally: measurement at the Henneke lab --> children's clinic (Sabine)
• optionally: measurement at Imaging Facility in the medicin clinic --> searching for authorized contact-persons

Wiki

• carry over the inofficial wiki content to the official one (Robert und Philipp).
• final report about the whole project (everyone)

part-order

• ask for the ATG orders (need to send the plasmid again?) (Philipp)
• obscurities at order BCR-transmembraneregion --> control and correction(Kathrin)
• primer-order to synthesize signalpeptide by PCR (Kathrin)

control of DNA-Origami binding to cell-surface

• add wash-steps after addition of DNA-origami
• cells should keep in Mg2+ for measurement
• Eventuell könnte man die Messung auch als ELISA durchführen --> immobilisierter AK --> daran bindet das DNA Origami --> 1. die an bestimmte Oligos gekoppelten Fluorophoren können dann detektiert werden --> 2. Man könnte auch Oligos mit Biotin für die DNA Origamis verwenden (sind im Labor vorhanden) und über Streptavidin und ein gekoppeltes Enzym eine Enzymreaktion auslösen
• Kontrolle ob der monoklonale AK an das DNA Origami binden kann (Messung im AFM) --> Daniel anrufen (Simone)

Sept. 24th. 2008
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Sept. 29th 2008
...

Oct. 7th 2008'
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Oct. 17th 2008

attendees:
Philipp Mappes, Katja Arndt, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib

Final report

• structure equal to a scientific paper containing the parts Abstract, Introduction, Materials and Methods, Results
• fusion of all sections we worked in: Origami, AFM, FACS, CA²⁺/Fura measuremt, cell cultures, modeling, ethics
• quotation of literature through autor and year of publication in the text (Max Muster, 2007), full quotation in references in the end
• deadline Friday Oct. 24th 2008

official wikipediasite

• continue the labjournal on the official page only
• not too much linking: maintenance of the flow of information
• all embedded files should start with "Freiburg2008"

iGEM parts

• uploading the parts starting on Monday Oct. 20th 2008

Oct. 21th 2008

attendees:
Moritz Busaker, Philipp Mappes, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib, Kathrin Pieper, Simone Weber

Cloning:
At least - All the constructes are cloned!!!

construcs:

pMA - singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - X – pMA

X :

• bla1(1/2 beta-Lactamase)-
  
• bla2 (1/2 beta-Lactamase) –
  
• split-linker-C-CFP(Cerutan) –
  
• N-CFP –
  
• split-linker-C-GFP (split venus)–
  
• N-GFP –
  
• luciferase 1 (58) –
  
• luciferase 2 (57) –
  

Next steps:
1) We want to test, if the transmembran region really is located in the membrane
Therefore we want to clone a constructe which expresses a YFP on the cytoplasmic side of the receptor.
Construct:
- singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - split-linker - bla1 - YFP -

2) We also want to test, if we able to bringe the receptors together by using a NIP coupled peptide.
Therefore we need the peptides from Schamels group -> Norman will ask them.

3) Origami-We want to try if the Origami are able to bring two or more receptors together.
Have to produce new Origami -> Michael and Norman

Next meeting: Oct. 23th 2008