Transcription factors

The syn. trans. factor system designed by caroline & david. cite memory.

• modularity
• extensibility

The laboratory of Dr. Pamela Silver at Harvard developed synthetic transcription factors for the construction of a novel “memory device” in Saccharomyces cerevisiae. While the details of this device are not essential to understanding our own gene network, it is important to discuss the significance of these transcription factors to our design. A transcription factor is composed of a binding domain, which targets the protein either an activation domain or a repression domain, which

Transcription factors are composed of a standard activation or repression domain linked to a variable binding domain. The binding domain is chosen such that it matches. Other features of these . A handful of these parts were available in the Registry, but we found it necessary to

The Biofusion Standard

Compared to other fields in biology, synthetic biology devotes a considerable amount of attention towards the standardization of parts and of practice. The inspiration for such a focus stems from similar concepts in engineering, which depends upon well-characterized systems for the design of complex systems that behave reliably according to predictions. Indeed, as readers of this wiki may be well aware, the iGEM competition focuses on the use of the Biobrick standard, which is an idempotent approach to cloning recombinant DNA. The Biobrick standard constitutes a conserved sequence of sites that envelop a genetic part, whether it be a coding gene, a promoter, or some other functional piece of DNA.

Our synthetic transcription factors were designed according to a

The differences between the two standards are minimal. In fact, the method of ligating two parts proves to be exactly the same, regardless of whether the part is destined to become a prefix or a suffix to another. Such a result is a consequence of the fact that the two standards share a similar However,

Modularity – in order to modify the design to limit the expression of a given gene of interest, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene

Stipulation: If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.

modifications to current scheme:

• Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
• alternatively, could consider having the promoter for construct Y produce an shRNA instead of a repressor protein
• need to consider the possibility for regulation on both levels of protein synthesis

Possible further modifications to these transcription factors include the use of different regulation domains,

Involves the consideration of multiple binding sites