Running an MFC Experiment

This page is intended as a comprehensive guide to completing a microbial fuel cell setup and running an experiment from start to finish.

Creating a Testing Environment

Begin 1-2 weeks prior to experiment

Constructing Fuel Cell Components

Materials (per fuel cell)

• 4" Polycarbonate Square Tube, 2" Outer Diameter
• 6" x 6" Polycarbonate Sheet, 1/4" Thick
• 4 Steel Fully Threaded Stud, 1/4"-20 Thread, 6" Length
• 8 Zinc Alloy Wing Flange Nut, 1/4"-20 Screw Size, 1" Wing Spread
• 1" x 1" Nafion® membrane, 0.180mm thick
• 1" x 1" Carbon felt, 0.25" thick
• 1" x 1" E-TEK ELAT™ GDE (platinum on carbon)
• 1' Titanium Grade 2 Wire .046" Diameter
• Teflon Tape, 1/4" Width
• 5" x 2.5" Silicone Sheet
• Silicone Glue
• Spiral Point Tap 1/4"-28
• 8 Plastic Luer Lock Coupling Nylon, Female to Male Thread, 1/4"-28

Procedure

1. Mill Polycarbonate
   • Cut polycarbonate sheet into 4 equal 3" x 3" pieces
   • Drill four 3/8" holes through each piece, 1 per corner, indented 5mm from both sides

1. • Cut polycarbonate tube into two equal 2" halves
   • Drill four 1/4" holes through each half in configuration shown

1. • Tap each hole with 1/4" -28 spiral tap

1. Glue Chambers (repeat for each half)
   • Center tube on endplate by marking plate with 'X' from corner to corner
   • Squirt 2mm thick line of silicone on edge of tube (edge furthest from holes)
   • Press tube firmly against marked location on endplate
   • Quickly spread excess silicone along edge
   • Let stand 24h to harden

1. Construct Gaskets
   • Cut silicone sheet into two equal 2.25" x 2.25" pieces
   • Cut out centered inner squares in each piece, 1.75" x 1.75"
   • Using inner squares, cut two 'O' rings, inner diameter 1/4", outer diameter 1/2"

1. Construct Electrodes

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1. Seal Injection Ports

Setup of Digital Multimeter

Controlling the DMM with LabView™

Experiment Preparation

Begin 1 day prior to experiment

Assembling Chambers

Solutions Prep

Chamber media

• 5.844 g/L 100mM NaCl
• 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

• 2.918 g/L Monosodium phosphate, monohydrate
• 4.095 g/L Disodium phosphate, anhydrous
• 5.844 g/L 100mM NaCl

7.0 pH

Gas Tubing Assembly

Growing Strains

Runtime

Begin 2 hours prior to experiment

Bacteria

Fuel Cells

Injections/Variables

Clean Up