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Team:Davidson-Missouri Western/New Parts Contributed to the Registry
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Revision as of 22:03, 29 October 2008 by Macampbell (Talk | contribs)
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The Davidson-Missouri Western team contributed 105 new parts to the Registry of Standard Biological Parts, including several novel hybrid promoters which have been designed to be dually-regulated, testing constructs, and selected intermediate parts. The new basic parts were sequence verified before continuing the building process to ensure against mutations.
| Number | Type | Description | Size | |
|---|---|---|---|---|
| BBa_K09100 | Composite | Receiver for AHL and Outputs GFP when AHL is present | 1946 bp | |
| BBa_K091001 | Coding | LsrR gene | 954 | |
| BBa_K091002 | Coding | LsrK gene | 1593 | |
| BBa_K091100 | Regulatory | pLac_lux hybrid promoter | 74 | |
| BBa_K091101 | Regulatory | pTet_Lac hybrid promoter | 83 | |
| BBa_K091104 | Regulatory | Mnt/LacI Hybrid Promoter | 87 | |
| BBa_K091105 | Regulatory | Mnt/TetR Hybrid Promoter | 98 | |
| BBa_K091106 | Regulatory | LsrA/cI hybrid promoter | 141 | |
| BBa_K091107 | Regulatory | Lux-cI hybrid promoter | 57 | |
| BBa_K091109 | Coding | LuxS | 516 | |
| BBa_K091110 | Regulatory | LacI Promoter | 56 | |
| BBa_K091111 | Regulatory | LacIQ promoter | 56 | |
| BBa_K091112 | Regulatory | LacIQ1 promoter | 56 | |
| BBa_K091114 | Regulatory | LsrAR Promoter | 248 | |
| BBa_K091115 | Regulatory | LsrR Promoter | 100 | |
| BBa_K091116 | Regulatory | LsrA Promoter | 126 | |
| BBa_K091117 | Regulatory | pLas promoter | 126 | |
| BBa_K091118 | Intermediate | Codes for LasR and LasR regulated GFP | 1985 | |
| BBa_K091119 | Intermediate | LacI protein generator with a pTet promoter | 74 | |
| BBa_K091120 | Intermediate | pTet regulated production of LacI protein | 1163 | |
| BBa_K091121 | Coding | LacI wild-type gene | 1083 | |
| BBa_K091122 | Coding | LacI_I12 protein | 1083 | |
| BBa_K091123 | Composite | LacI_I12 protein regulated by pTet | 1163 | |
| BBa_K091125 | Composite | GFP generator regulated by pTet | 938 | |
| BBa_K091126 | Composite | pTet regulated production of LacI_X86 | 1163 | |
| BBa_K091127 | Coding | LacI_I12_X86 | 1083 | |
| BBa_K091128 | Composite | pTet regulation of the LacI_I12_X86 protein | 1163 | |
| BBa_K091131 | Generator | pLacIQ1 controlled GFP generator | 940 | |
| BBa_K091132 | Composite | pMnt/Tet+RBS+LuxI | 767 | |
| BBa_K091133 | Composite | pBAD+RBS+TetR | 841 | |
| BBa_K091134 | Device | Las Receiver | 2048 | |
| BBa_K091136 | Signalling | Las Sender Testing Device | 670 | |
| BBa_K091137 | Coding | RBS+Mnt+TT | 468 | |
| BBa_K091138 | Composite | RBS+lsrR | 972 | |
| BBa_K091139 | Composite | RBS+lsrR+TT | 1109 | |
| BBa_K091140 | Composite | plsrA/cI+RBS+luxI | 810 | |
| BBa_K091141 | Composite | RBS+Mnt+TT | 468 | |
| BBa_K091142 | Intermediate | plsrA/cI+RBS+luxI+RBS+Mnt+TT | 1286 | |
| BBa_K091143 | Regulatory | plasR/cI Hybrid Promoter | 164 | |
| BBa_K091144 | Composite | pMnt/Tet+RBS+LuxI+RBS+cI+TT | 1671 | |
| BBa_K091145 | Composite | pBAD+RBS+TetR+TT | 978 | |
| BBa_K091146 | Regulatory | pLas+ Lux- Promoter | 126 | |
| BBa_K091147 | Composite | pBAD+RBS+Mnt+TT | 606 | |
| BBa_K091148 | Generator | pLas' Promoter Testing Construct A | 1997 | |
| BBa_K091149 | Signalling | pLas' Testing Construct B | 1997 | |
| BBa_K091150 | Signalling | pLas' Testing Construct C | 2804 | |
| BBa_K091151 | Signalling | pLas' Testing Construct D | 2761 | |
| BBa_K091152 | Signalling | pLas' Testing Construct D | 2804 | |
| BBa_K091153 | Signalling | Standard RBS with LuxS | 534 | |
| BBa_K091154 | Composite | RBS+luxI+RBS+Mnt+TT | 1137 | |
| BBa_K091155 | Intermediate | plasR/cI+RBS+luxI+RBS+Mnt+TT | 1309 | |
| BBa_K091156 | Regulatory | pLux | 55 | |
| BBa_K091157 | Regulatory | pLux+ Las- Promoter | 55 | |
| BBa_K091158 | Composite | RBS+lsrK | 1611 | |
| BBa_K091159 | Composite | pBAD+RBS+lsrK | 1749 | |
| BBa_K091160 | Composite | pMnt/Tet-RBS-GFP-TT | 982 | |
| BBa_K091161 | Signalling | RBS+LasI+RBS+Mnt+TT | 1103 | |
| BBa_K091162 | Signalling | Promoter+RBS+LasI+RBS+Mnt+TT | 1168 | |
| BBa_K091163 | Signalling | RBS+LuxS+RBS+Mnt+TT | 1010 | |
| BBa_K091164 | Signalling | Promoter+RBS+LuxS+RBS+Mnt+TT | 1075 | |
| BBa_K091165 | Reporter | pLux/cI+RBS+GFP+TT | 941 | |
| BBa_K091166 | Signalling | pBAD-RBS-luxR | 937 | |
| BBa_K091167 | Signalling | RBS+LuxI+TT | 798 | |
| BBa_K091168 | Composite | pLac/Mnt+RBS+LuxS+RBS+cI+TT | 1533 | |
| BBa_K091169 | Composite | plasR/cI+E0240(GFP) | 1048 | |
| BBa_K091170 | Composite | plsrA/cI+E0240(GFP) | 1025 | |
| BBa_K091171 | Composite | pBAD+RBS+cI+TT | 1034 | |
| BBa_K091172 | Intermediate | lsr system supporting genes | 2866 | |
| BBa_K091173 | Device | pLsr/cI+RBS+luxI+RBS+Mnt+TT+pTet/Mnt+RBS+luxI+RBS+cI+TT | 2965 | |
| BBa_K091174 | Device | pBAD-RBS-TetR-TT-pBAD-RBS-LsrK-RBS-LsrR-TT | 3852 | |
| BBa_K091175 | Composite | pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT | 1968 | |
| BBa_K091176 | Composite | pBAD-RBS-Mnt-TT-pMnt/tetR-RBS-GFP-TT | 1596 | |
| BBa_K091177 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT | 1968 | |
| BBa_K091178 | Composite | pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT | 1596 | |
| BBa_K091179 | Signalling | LuxR receiver and pBAD promoter | 1074 | |
| BBa_K091180 | Composite | pLac-RBS-LasR-TT | 999 | |
| BBa_K091181 | Composite | pBad-RBS-tetR-TT-placI/pI-RBS-lasR-TT | 1985 | |
| BBa_K091182 | Composite | pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT | 2988 | |
| BBa_K091183 | Signalling | pBAD+RBS+LuxR+RBS+cI lam+TT | 1841 | |
| BBa_K091184 | Regulatory | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT | 2616 | |
| BBa_K091185 | Composite | LacI_I12+TT | 1220 | |
| BBa_K091186 | Composite | pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | 2528 | |
| BBa_K091188 | Composite | pMnt/lac+RBS+lasI+RBS+cI+TT | 1626 | |
| BBa_K091190 | Composite | plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT | 2802 | |
| BBa_K091191 | Composite | pBAD+RBS+luxS+TT | 809 | |
| BBa_K091192 | Composite | pBAD+RBS+lasI+TT | 807 | |
| BBa_K091194 | Reporter | Promoter testing construct | 2023 | |
| BBa_K091195 | Reporter | Promoter testing construct | 1983 | |
| BBa_K091196 | Reporter | Promoter testing construct | 2790 | |
| BBa_K091197 | Intermediate | plsrAcI testing construct with GFP | 3899 | |
| BBa_K091198 | Intermediate | plasRcI testing construct | 2055 | |
| BBa_K091199 | Intermediate | plasR/cI overall testing construct | 3097 | |
| BBa_K091200 | Intermediate | plsrA/cI testing construct with lambda cI repression | 2067 | |
| BBa_K091201 | Intermediate | plsrAcI overall testing construct | 4941 | |
| BBa_K091202 | Intermediate | plasRcI testing construct with lambda cI repression | 2090 | |
| BBa_K091203 | Device | pBAD-RBS-LuxR-RBS-LacI(I12)-TT | 2183 | |
| BBa_K091204 | Coding | Prom-LuxR-TT | 979 | |
| BBa_K091205 | Coding | Prom-LasR-TT | 979 | |
| BBa_K091206 | Coding | Prom-LuxR-TT-Prom-LasR-TT | 1966 | |
| BBa_S03967 | Intermediate | RBS-LasI | 627 | |
| BBa_S03968 | Intermediate | RBS-LuxS | 534 | |
| BBa_S03969 | Intermediate | pMnt/Lac-RBS-GFP-TT | 971 | |
| BBa_S03979 | Intermediate | RBS-LuxS-RBS-cI-TT | 1438 | |
| BBa_S03980 | Intermediate | pBAD-RBS-LacI-TT | 1446 | |
| BBa_S04058 | Intermediate | RBS-LacI12-TT | 1238 |
Safety considerations: To the best of our knowledge, the new parts above pose no threats to researcher, public, or environmental safety. Student researchers are instructed by faculty mentors in the use of best practices in laboratory procedures when handling bacteria and reagents.
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