Team:Hawaii/Notebook/2008-10-27
From 2008.igem.org
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Things we did today
Wetlab work
Plasmid prep of BBpRL1383a
- Grace
- Used Qiaprep Spin Miniprep Kit to prep the rest of the BBpRL1383a liquid cultures.
Cryostocked BBpRL1383a
- Grace
- Cryostocked 10/22 culture of DH5α w/ BBpRL1383a
Restriction digest
- Grace
- 6 hour XbaI/SpeI digest (for pretty picture):
- nir, nir+rbs, slr, pilA, slr+GFPf, GFPf
- Overnight HindIII/BamHI digest:
- J33207
Triparental conjugation
- Grace
- Checked on triparental conjugation cultures from 10/13. No growth was observed in any of the cultures.
- E. coli were grown at too low antibiotic concentration (sp2.5). Cells probably lost plasmid during growth, before conjugation. Will repeat triparental conjugation on Wednesday.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]