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Team:Edinburgh/GBS1
From 2008.igem.org
Contents |
GBS1
For better alignment see .txt file
AMINO ACID SEQUENCE
MATvTASSNFvSRTSLFNNHGASSCSDVAQITLKGQSlTHCGLRSFNMvD
NLQRRSQAKPVSAKSSKRSSKVKTAGKIvCEKGMSvIFIGAEVGPWSKTG
GLGDVLGGLPPALAARGHRvMTICPRYDQYKDAWDTCVvVQIKVGDKVEN
VRFFHCYKRGVDRVFVDHPIFLAKVvGKTGSKIYGPITGVDYNDNQLRFS
llCQAALEAPQVlNlNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQ
SRGVYMNAKvVFCIHNIAYQGRFAFDDYSLLNlPISFKSSFDFMDGYEKP
VKGRKINWMKAAIlEAHRVLTVSPYYAQELISGVDRGvElHKYLRMKTVS
GIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPlIKEALQAAVGLPvDRD
VPVIGFIGRlEEQKGSDILvEAISKFMGLNVQMVILGTGKKKMEAQILEL
EEKFPGKAVGvAKFNvPlAHMITAGADFIIVPSRFEPCGLIQlHAMRYGT
VPIvASTGGLvDTvKDGYTGFHIGRFNVKCEVvDPDDvIATAKAvTRAVA
VYGTSAMQEMVKNCMDQDFSWKGPARlWEKVLlSLNvAGSEAGTEGEEIA
PlAKENVATP*
DNA SEQUENCE
atggcaactgtgactgcttcttctaactttgtgtcaagaacttcactttt 50
taccgttgacactgacgaagaagattgaaacacagttcttgaagtgaaaa
caacaatcatggtgcttcttcatgctctgatgtcgctcagattaccttaa 100
gttgttagtaccacgaagaagtacgagactacagcgagtctaatggaatt
aaggccaatccttgactcattgtgggttaaggtcattcaacatggtggat 150
ttccggttaggaactgagtaacacccaattccagtaagttgtaccaccta
aaccttcagaggagatctcaagctaaacctgtttctgctaaatcctcaaa 200
ttggaagtctcctctagagttcgatttggacaaagacgatttaggagttt
gagatcttctaaagttaagactgctggtaagattgtgtgtgagaaaggaa 250
ctctagaagatttcaattctgacgaccattctaacacacactctttcctt
tgtctgtgatttttattggagctgaagttggtccatggagtaaaactggt 300
acagacactaaaaataacctcgacttcaaccaggtacctcattttgacca
ggtcttggtgatgttctcggtggtctacctccagctcttgctgctagagg 350
ccagaaccactacaagagccaccagatggaggtcgagaacgacgatctcc
ccaccgtgtgatgacaatttgtcctcggtatgaccaatataaagatgctt 400
ggtggcacactactgttaaacaggagccatactggttatatttctacgaa
gggacacttgtgttgtggttcagatcaaagttggggataaagttgagaat 450
ccctgtgaacacaacaccaagtctagtttcaacccctatttcaactctta
gttcgtttcttccattgctacaaacgaggagttgatcgtgtctttgttga 500
caagcaaagaaggtaacgatgtttgctcctcaactagcacagaaacaact
ccatccaatctttcttgctaaggttgtgggcaaaacaggatccaaaatct 550
ggtaggttagaaagaacgattccaacacccgttttgtcctaggttttaga
atggtcctataactggagtagactacaatgacaaccaactccggttcagt 600
taccaggatattgacctcatctgatgttactgttggttgaggccaagtca
ttgttgtgtcaggctgctcttgaggcaccacaggttctgaacctgaacag 650
aacaacacagtccgacgagaactccgtggtgtccaagacttggacttgtc
cagcaagtacttctctggaccatatggtgaagatgtagtctttgttgcca 700
gtcgttcatgaagagacctggtataccacttctacatcagaaacaacggt
atgactggcacactgctctacttccatgttacctcaaatctatgtatcaa 750
tactgaccgtgtgacgagatgaaggtacaatggagtttagatacatagtt
tcccgcggagtctacatgaatgcaaaggtggtcttctgcattcacaacat 800
agggcgcctcagatgtacttacgtttccaccagaagacgtaagtgttgta
agcctaccagggaagatttgcctttgatgactattcccttctcaacttgc 850
tcggatggtcccttctaaacggaaactactgataagggaagagttgaacg
ccatcagctttaaaagttctttcgacttcatggacgggtatgaaaagcca 900
ggtagtcgaaattttcaagaaagctgaagtacctgcccatacttttcggt
gtaaaaggacggaaaattaactggatgaaggctgcaattctggaagctca 950
cattttcctgccttttaattgacctacttccgacgttaagaccttcgagt
ccgtgtcttaacagttagtccatactacgctcaagaactcatctctggag 1000
ggcacagaattgtcaatcaggtatgatgcgagttcttgagtagagacctc
v
ttgatagaggcgtggaattgcataaatatcttcgaatgaaaacagtttcc 1050
aactatctccgcaccttaacgtatttatagaagcttacttttgtcaaagg
ggaattattaatggaatggatgttcaagaatggaacccgtctactgacaa 1100
ccttaataattaccttacctacaagttcttaccttgggcagatgactgtt
gtacatcgatatcaaatacgatattaccactgttacagatgctaaaccat 1150
catgtagctatagtttatgctataatggtgacaatgtctacgatttggta
tgatcaaagaagcacttcaggctgctgttggacttcccgtggacagggat 1200
actagtttcttcgtgaagtccgacgacaacctgaagggcacctgtcccta
gtcccggttatcggtttcatagggagattggaggagcagaagggttctga 1250
cagggccaatagccaaagtatccctctaacctcctcgtcttcccaagact
tattctagtggaagctatttccaagttcatggggctcaatgttcagatgg 1300
ataagatcaccttcgataaaggttcaagtaccccgagttacaagtctacc
ttatccttgggactggaaagaagaagatggaggctcagattcttgaacta 1350
aataggaaccctgacctttcttcttctacctccgagtctaagaacttgat
gaagagaagttcccagggaaggcggttggagtggcgaaattcaacgtgcc 1400
cttctcttcaagggtcccttccgccaacctcaccgctttaagttgcacgg
attggctcatatgatcactgctggagctgacttcatcattgtcccaagca 1450
taaccgagtatactagtgacgacctcgactgaagtagtaacagggttcgt
ggtttgagccgtgtggtctcattcagctgcacgcaatgagatatggaacc 1500
ccaaactcggcacaccagagtaagtcgacgtgcgttactctataccttgg
gtccctattgtggcatctactggtggacttgtggacactgtgaaagatgg 1550
cagggataacaccgtagatgaccacctgaacacctgtgacactttctacc
ctacacaggtttccacattggaagattcaacgtcaagtgtgaagttgtgg 1600
gatgtgtccaaaggtgtaaccttctaagttgcagttcacacttcaacacc
atccagatgatgtgatagcaacagcaaaggctgtgacaagagccgttgca 1650
taggtctactacactatcgttgtcgtttccgacactgttctcggcaacgt
gtatatggaacatccgcaatgcaagaaatggtcaagaactgcatggacca 1700
catataccttgtaggcgttacgttctttaccagttcttgacgtacctggt
agacttctcctggaagggacctgcgaggttgtgggagaaggtactattgt 1750
tctgaagaggaccttccctggacgctccaacaccctcttccatgataaca
cccttaatgtggcgggaagtgaagccggaaccgagggtgaagagatagct 1800
gggaattacaccgcccttcacttcggccttggctcccacttctctatcga
cctctggccaaggagaacgtagcgacgccgtga 1833
ggagaccggttcctcttgcatcgctgcggcact
No forbidden restriction sites!
BIOBRICKTM PRIMERS
Forward: CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT (54ºC)
Reverse: CGT t actagt a TTA TTA CGG CGT CGC TAC G (52ºC)
