Team:Chiba/Calendar-Home/19 September 2008

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18 September 2008 <|> 20 September 2008

Laboratory work

Team:Communication

-->(?/9) Gel Check

300px
Sample Name [http://partsregistry.org/Part:BBa_J04500 J04500][http://partsregistry.org/Part:BBa_R0010 R0010][http://partsregistry.org/Part:BBa_C0161 C0161]Cin
Sample DNA(μL) 30309020
Loading Dye(μL) 66184
TOTAL(μL) 363610824
J04500' (Plac+RBS)

300px R0010 (Plac)

300px C0161 (RBS+Lux)

300px C0261 (Lux+LVA)


--->Gel Extract


(3 wells for 1 sample)

  • note:ADB buffer,
  • left at 37°C,for 6 hours
--->Zymo Clean
  • elution
    • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]-->27μL
    • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161]-->11μL

--->Gel Check

--->Ligation
Sample Name insert+vectorinsertvector
[http://partsregistry.org/Part:BBa_C0161 C0161](μL) 33-
[http://partsregistry.org/Part:BBa_J04500 J04500](μL) 4.5-4.5
ligase(μL) 111
Buffer(invitrogen)(μL) 322
NFW(μL) 3.542.5
TOTAL(μL) 151010
--->Transformed above ligation onto a LB-amp plate.
  • note
    • competent cell:XL10GOLD
    • DNA:1μL,SOC:120μL

--->(20/9)Mini prep


  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature

30°C and Room Temparature

Team:Output


colony PCR
insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](×16)(marker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004](×5)(marker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005](×7)(marker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006](×5)(marker:Kan)

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