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Team:Hawaii/Notebook/2008-10-24
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Prep for sequencing BB1
- Grace
- ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing
Plasmid prep of parts to send to iGEM
- Grace
| Plasmid | Concentration |
|---|---|
| nir | |
| slr | |
| pilA | |
| GFPf | |
| nir+rbs | |
| slr+GFPf | |
| BBpRL1383a #1 from sp100 plate |
Antibiotic test
- Grace
| Plate | Colony | Growth? | Blue? |
|---|---|---|---|
| LB+amp100 | sp2.5 blue | No | No |
| sp100 #1 | No | No | |
| sp100 #2 | No | No | |
| LB | sp2.5 blue | Yes | Yes, ~20 |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No | |
| LB+sp100 | sp2.5 blue | No | No |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No |
- Observations collected 19 hours after initial plating.
Dry Lab Work
Analyzed sequencing results
Krystle
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
