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Things we did today

Wetlab work

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.

Grace

• Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
• Ran on gel to verify plasmid

• ~2ng/μl
• The prep kit yields really clean preps in ~1 hour. :D!

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.

Margaret

Construction of BB-pRL1383a/K125820

Krystle

• Transformation of overnight ligation products
• Plated onto LB sp₁₀₀ plates

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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