Team:BrownTwo/Limiter/altdesign

Alternative construction and design possiblities

• up-limiter
• dual limiter
• limiter device as a regulatory bridge between two pathways
• killswitch
• Smolke’s gal2 knockout scheme for using the GAL1 promoter
• Finding additional inducible/repressible promoters to use in the system

• Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)

In order to modify our limiter to regulate an endogenous gene network, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene. A handful of these parts were available in the Registry, but we found it necessary to

Modularity –

Stipulation: If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.

modifications to current scheme:

Possible further modifications to these transcription factors include the use of different regulation domains,

Involves the consideration of multiple binding sites