Hung, Darius
- Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
- Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
- Gel extraction of E7 bands (around 2kb) to remove all the oligonucleotides.
- Purification for E7 after extracted.
- Digestion of GFP by EcoRI and PstI. Incubation for 3 hours.
- Digestion of purified E7 by EcoRI and PstI. Incubation for 3 hours.
- Gel electrophoresis and gel extraction for GFP to get the empty plasmid vector (around 2kb).
- PCR purification for digested E7 to remove all the enzymes, buffers and small nucleotides.
- Ligation of empty plasmid (from GFP) and E7 insert.
- Transformation and cloning into homemade top10 cells.
Lu Chao, Hung
transformation and cell cloning of LacI-GFP and LacI-RFP again.
Lu Chao
inoculation of LacI-RBS cells.
Choon Kit
documentation of lab protocols.
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