• Team Toxipop did extensive research in April and May once our project was decided. Our research extended throughout the summer as different problems and ideas presented themselves. The following papers guided us throughout the process. They include information about the Cell Lysis Cassette and the individual genes that make up the composite part. We also looked into previous water toxin detection systems. This included looking at current products on the market as well as scientific literature that provided valuable information about different reporting systems.

Papers

"Use of Lambda Phage S and R Gene Products in an Inducible Lysis System for Vibrio cholerae- and Salmonella enterica Serovar Typhimurium-Based DNA Vaccine Delivery Systems" VIVEK JAIN AND JOHN J. MEKALANOS, Harvard Medical School

“Mutational analysis of bacteriophage lambda lysis gene S” , R RAAB, G NEAL, J GARRETT, R GRIMAILA, R FUSSELMAN, AND R YOUNG

“Development of a Simple Cell Lysis Method for Recombinant DNA Using Bacteriophage Lamda Lysis Genes”, JANG, BOYUN, YUNA JUNG, AND DONGBIN LIM

“Cell lysis by induction of cloned lambda lysis genes” -GARRETT J, FUSSELMAN R, HISE J, CHIOU L, SMITH-GRILLO D, SCHULZ J, YOUNG R

E. coli Information Database: Institute for Biomolecular Design, University of Calgary

“Phi X174 E complements lambda S and R dysfunction for host cell lysis” - W D ROOF AND R YOUNG

US patent (100/17300) Method to Eliminate Transient Spikes on Fluidic Flow Caused by Inadequate Current and Voltage Changes, by Caliper Life Sciences (2002)

"Holins Kill Without Warning" PNAS ANGELIKA GRUNDLING, MICHAEL D. MANSON, RY YOUNG

"HOLINS: The Protein Clocks of Bacteriophage Infections" Annual Reviews: A Nonprofit Scientific Publisher - ING-NANG WANG, DAVID L. SMITH, RY YOUNG