Team:Chiba/Calendar-Home/10 September 2008

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9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->Colony Count

  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


-->Gel Check

Chiba-0910.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
Marker
  1. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  2. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  3. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  4. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  5. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  6. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  7. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> Bad
  8. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  9. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  10. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
Marker
  1. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  2. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  3. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  4. [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] ---control -> OK
Marker


--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

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