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Team:The University of Alberta/23 September 2008
From 2008.igem.org
Digest 21+22+23 with Xba/Pst
Run and purify J6+IP and 21+22+23
Ligate J6/I0500 with 21+22+23, 21+23, 22+24, 24+25.
Ligate ETFB to ETFA
Ligate 24+25 with 21+22+23