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Team:University of Ottawa/18 July 2008
From 2008.igem.org
Contents |
Today in the Lab
Matt
- PCR Amplification
- A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
- Transformation
- The transformation was a success with both plates streaked growing numerous colonies.
- For miniprep on Monday, I think I will choose 5 colonies per plate.
- An inoculation will be performed today into liquid media so that a miniprep can be performed.
Dan
- PCR
- PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
- Digestion
- 1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
- Ligation
- Was performed overnight.
Tammy
- Gel Electrophoresis of pDR197::AtCKX2 Digestion Products
- Total plasmid size = 7.9 kb
- AtCKX2 insert size = 1.67 kb (NM_127508)
- pDR197 vector only = 6.23 kb
- Glycerol Stock confirmed pDR197::AtCKX2
- Stocked PURE I and 1:10 II
- 0.5 mL Sample
- 0.5 ml LB + Glucose
