Edinburgh/4 August 2008

From 2008.igem.org

Edinburgh iGEM 2008

June
M	T	W	T	F	S	S
						[http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16]	[http://2008.igem.org/Edinburgh/17_June_2008 17]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18]	[http://2008.igem.org/Edinburgh/19_June_2008 19]	[http://2008.igem.org/Edinburgh/20_June_2008 20]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23]	[http://2008.igem.org/Edinburgh/24_June_2008 24]	[http://2008.igem.org/Edinburgh/25_June_2008 25]	[http://2008.igem.org/Edinburgh/26_June_2008 26]	[http://2008.igem.org/Edinburgh/27_June_2008 27]	[http://2008.igem.org/Edinburgh/28_June_2008 28]	[http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/Edinburgh/1_July_2008 1]	[http://2008.igem.org/Edinburgh/2_July_2008 2]	[http://2008.igem.org/Edinburgh/3_July_2008 3]	[http://2008.igem.org/Edinburgh/4_July_2008 4]	[http://2008.igem.org/Edinburgh/5_July_2008 5]	[http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7]	[http://2008.igem.org/Edinburgh/8_July_2008 8]	[http://2008.igem.org/Edinburgh/9_July_2008 9]	[http://2008.igem.org/Edinburgh/10_July_2008 10]	[http://2008.igem.org/Edinburgh/11_July_2008 11]	[http://2008.igem.org/Edinburgh/12_July_2008 12]	[http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14]	[http://2008.igem.org/Edinburgh/15_July_2008 15]	[http://2008.igem.org/Edinburgh/16_July_2008 16]	[http://2008.igem.org/Edinburgh/17_July_2008 17]	[http://2008.igem.org/Edinburgh/18_July_2008 18]	[http://2008.igem.org/Edinburgh/19_July_2008 19]	[http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21]	[http://2008.igem.org/Edinburgh/22_July_2008 22]	[http://2008.igem.org/Edinburgh/23_July_2008 23]	[http://2008.igem.org/Edinburgh/24_July_2008 24]	[http://2008.igem.org/Edinburgh/25_July_2008 25]	[http://2008.igem.org/Edinburgh/26_July_2008 26]	[http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28]	[http://2008.igem.org/Edinburgh/29_July_2008 29]	[http://2008.igem.org/Edinburgh/30_July_2008 30]	[http://2008.igem.org/Edinburgh/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Edinburgh/1_August_2008 1]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2]	[http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4]	[http://2008.igem.org/Edinburgh/5_August_2008 5]	[http://2008.igem.org/Edinburgh/6_August_2008 6]	[http://2008.igem.org/Edinburgh/7_August_2008 7]	[http://2008.igem.org/Edinburgh/8_August_2008 8]	[http://2008.igem.org/Edinburgh/9_August_2008 9]	[http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11]	[http://2008.igem.org/Edinburgh/12_August_2008 12]	[http://2008.igem.org/Edinburgh/13_August_2008 13]	[http://2008.igem.org/Edinburgh/14_August_2008 14]	[http://2008.igem.org/Edinburgh/15_August_2008 15]	[http://2008.igem.org/Edinburgh/16_August_2008 16]	[http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18]	[http://2008.igem.org/Edinburgh/19_August_2008 19]	[http://2008.igem.org/Edinburgh/20_August_2008 20]	[http://2008.igem.org/Edinburgh/21_August_2008 21]	[http://2008.igem.org/Edinburgh/22_August_2008 22]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25]	[http://2008.igem.org/Edinburgh/26_August_2008 26]	[http://2008.igem.org/Edinburgh/27_August_2008 27]	[http://2008.igem.org/Edinburgh/28_August_2008 28]	[http://2008.igem.org/Edinburgh/29_August_2008 29]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]

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Week 8

Monday 4 August 08

Made 17 minipreps from colonies on plates 83-86: M80-83 from plate 83 (L26: pSB1A2+rbs+appY), M84-85 from plate 84 (L27: pSB1A2+rbs+crtB), M86-90 from plate 85 (L28: pSB1A2+cenA) and M91-96 from plate 86 (L29: pSB1A2+cex). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
Third glgC mutagenesis performed by MABEL on BABEL2+glgC-mut1,2 (since pSB1A2+glgC-mut1,2 appears to have failed): P50 (Yan)
P50 and double digestions of M80-M96 were run on gel 36. Results were good for M80-82 (pSB1A2+rbs+appY), M84-85 (pSB1A2+rbs+crtB), M92 (pSB1A2+cex) and P50 (glgC-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+cenA). (Yan, OG)
Sequencing results back today indicate that M50 is pSB1A2+rbs+crtI, M63 is pSB1A2+rbs+crtE and M67 is pSB1A2+rbs+crtB. (AH)

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