Team:Hawaii/Notebook/2008-06-11

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Things we did today

Wetlab work

Tri-Parental Conjugation Preparation

Krystle
  • Innoculated E.coli into 40mL SOB in Erlenmeyer flasks, placed in 37oC incubator @ 250rpm
  • Made 165mL BG-11 for BG-11 washes
  • See experiment for details.

Make more LB+Amp plates

Krystle

Thaw Test

Norman
  • take final picture of thawing post thawing (~1wk). Writeup discussion about the experiment. We have OK cryopreserved cells although it does not grow back very fast. For more thorough test we need to measure OD, as well as optimal conditions... (CO2 incubator!!!) Right now at least we know we have viable frozen cells.

Drylab Work

Standardized Notebook entries on the Wiki

Krystle

Fix Internal Wiki

Norman
fixed permission problems with wiki on internal prestinglab server. If you have trouble accessing it let me know.

Discussion

Quote of the Day

I'll just ask Grace later. She speaks Krystle-language, meaning we save a lot of time because she already understands that I think weird and will adjust her explanation accordingly - Krystle to Norman about designing primers


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