From 2008.igem.org
Things we did today
Wetlab work
PCR
- Grace
- nir and J33207 from plasmid preps
- Verified products on 1.2% agarose gel
Plasmid preps
- Grace
- Resuspended plasmid preps in TE buffer
- Verified preps on 1.2% agarose gel
Restriction digest
- Grace
- BB-pRL1383a and J33207 with XbaI and PstI
- nir with EcoRI and SpeI
- C0012 with EcoRI and PstI
Sequencing continued from 8-27
- Margaret
| construct
| quantity(ng/ul)
|
- Exo-Sap:OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6
- OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6.
- The omega interposon DNA was not submitted because there were multiple bands-->tomorrow I will work some more on this
- also submitted some of Grace's constructs
Colony PCR
- Margaret
-
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
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