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Team:Hawaii/Notebook/2008-09-28
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Ligation
- Margaret
- Ligation of promoter/rbs to rep into pSB1A3 (dephosphorylated)
- Ligation of pSB1A3 (dephosphorylated) to self
Transformation into DH5-alpha
- Margaret
- promoter/rbs to rep into pSB1A3 (dephosphorylated)
- pSB1A3 (dephosphorylated) to self ligation
- (+) pUC18
- (-) no plasmid
Culture DH5-alpha with plamids
- Margaret
- aadA (BB)with promoter/rbs/terminator
- aadA (pRL)with promoter/rbs/terminator
- rep
- oriV
- oriT (J33207)
- oriTr (J33207)
Drylab Work
Name of Task
- Margaret
- The sequence of rep (submitted 9/24) was verified using Seqman software
- region between 1550-1600 was not covered by primers, so an additional primer was designed and ordered. Will submit again for sequencing
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
