Alberta NINT/28 May 2008

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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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[[Alberta_NINT/27_May_2008 | < Previous entry]]
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[[Alberta_NINT/27_May_2008 | < Previous entry]] | [[Alberta_NINT/29_May_2008 | Next entry >]]
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lab work (SD):  
lab work (SD):  
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           Ran gel electrophoresis and cut out desired band.
           Ran gel electrophoresis and cut out desired band.
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[[Alberta_NINT/29_May_2008 | Next entry >]]
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lab work (WM):
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          Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI)
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          Transformed 2 ul into 100 ul XL1-B. 
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          Plated 150 ul on LB + kan35

Latest revision as of 21:38, 11 October 2008

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lab work (SD):

        Transformed XL1-B cells with the plasmids created 27/05/08.  
        K102002 had 170 colonies.  K102004 had 629 colonies.  K102000 negative control had 5 colonies.

lab work (JD):

         Carried out mini-preps on O/Ns
         Digested R0011 with SpeI+ PstI
         Ran gel electrophoresis and cut out desired band.

lab work (WM):

         Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI) 
         Transformed 2 ul into 100 ul XL1-B.  
         Plated 150 ul on LB + kan35