B22222

From 2008.igem.org

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     <td height="311" colspan="2" bgcolor="#03438A" class="subHeader STYLE5"><p class="STYLE25"><span class="STYLE12">Background</span><br />
     <td height="311" colspan="2" bgcolor="#03438A" class="subHeader STYLE5"><p class="STYLE25"><span class="STYLE12">Background</span><br />
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      There is no mono-culture in nature! And in industry,  coculture of species/strains are widely used to either improve productivity or  lower the cost. The manufacturing of Vitamin C in China, which has contributed to 60  percent of its world production, could serve as an excellent example to  validate the significance of coculture in industry. Thus to understand the  interactions between coexistent ecosystems will not only contribute to human’s  perception of nature but also to human practices in engineering.</p>
 
         <p class="STYLE25">The attempts to uncover the mechanics and complex  interrelations within natural microbial systems and quantitatively measurement  of environmental factors on system behavior often failed because of the  entangled intrinsic parameters and un-measurable population dynamics.</p>
         <p class="STYLE25">The attempts to uncover the mechanics and complex  interrelations within natural microbial systems and quantitatively measurement  of environmental factors on system behavior often failed because of the  entangled intrinsic parameters and un-measurable population dynamics.</p>
     <p class="STYLE25">We designed and constructed an ecosystem constituted  of two strains of E.coli, which could represent various biological  relationships along with the fluctuation of antibiotics concentration as the  environmental pressure and the inducing molecular such as IPTG and arabinose as  the regulating factors. </p></td>
     <p class="STYLE25">We designed and constructed an ecosystem constituted  of two strains of E.coli, which could represent various biological  relationships along with the fluctuation of antibiotics concentration as the  environmental pressure and the inducing molecular such as IPTG and arabinose as  the regulating factors. </p></td>
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     <td height="390" colspan="2" bgcolor="#03438A" class="subHeader"><table width="968" height="384" border="0" align="center">
     <td height="390" colspan="2" bgcolor="#03438A" class="subHeader"><table width="968" height="384" border="0" align="center">
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         <td width="318" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE8"><span class="STYLE10"><strong>Toggle switch</strong>-----toggle switch is a switch on the basis  of two mutually-repressive promoters, the product of each represses the express  of that of the other, and both the repressors could be deactivated in certain  conditions. And the state of the cell could be regulated by the change of the  culture variations.</span></p></td>
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         <td width="318" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE8"><span class="STYLE10"><strong>Convertable switch</strong>-----toggle switch is a switch on the basis  of two mutually-repressive promoters, the product of each represses the express  of that of the other, and both the repressors could be deactivated in certain  conditions. And the state of the cell could be regulated by the change of the  culture variations.</span></p></td>
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         <td width="319" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE11"><span class="STYLE4"><strong>Quoru
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         <td width="319" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE11"><span class="STYLE4"><strong>Quorum sensing</strong>-----That is the way how various bacteria “talk”  to each other. It is the mechanism ensures that certain genes will keep silent  before the cell density of the species pass a threshold. </span><img src="https://static.igem.org/mediawiki/2008/b/b3/F2620Parts.png" width="223" height="48" /></p></td>
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          m sensing</strong>-----Th
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          at is the way how various bacteria “talk”  to each other. It is the mechanism ensures that certain genes will keep silent  before the cell density of the species pass a threshold. </span><img src="https://static.igem.org/mediawiki/2008/b/b3/F2620Parts.png" width="223" height="48" /></p></td>
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         <td width="329" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE5"><span class="STYLE9"><strong>Prisoners’  Dilemma----</strong>It is the dilemma in which the two  suspects could either choose to cooperate with or betray each other. In  conditions when they could communicate freely with each other, they will cooperate,  which maximizes their benefits as a whole; while when they are inquisited separately,  they will both choose to betray one another to lower the risks of long sentence. </span></p></td>
         <td width="329" bordercolor="#03438A" bgcolor="#03438A"><p class="STYLE5"><span class="STYLE9"><strong>Prisoners’  Dilemma----</strong>It is the dilemma in which the two  suspects could either choose to cooperate with or betray each other. In  conditions when they could communicate freely with each other, they will cooperate,  which maximizes their benefits as a whole; while when they are inquisited separately,  they will both choose to betray one another to lower the risks of long sentence. </span></p></td>
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Revision as of 10:00, 29 October 2008

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The synthetic convertible ecosystem

Background

The attempts to uncover the mechanics and complex interrelations within natural microbial systems and quantitatively measurement of environmental factors on system behavior often failed because of the entangled intrinsic parameters and un-measurable population dynamics.

We designed and constructed an ecosystem constituted of two strains of E.coli, which could represent various biological relationships along with the fluctuation of antibiotics concentration as the environmental pressure and the inducing molecular such as IPTG and arabinose as the regulating factors.

The Tools

Convertable switch-----toggle switch is a switch on the basis of two mutually-repressive promoters, the product of each represses the express of that of the other, and both the repressors could be deactivated in certain conditions. And the state of the cell could be regulated by the change of the culture variations.

Quorum sensing-----That is the way how various bacteria “talk” to each other. It is the mechanism ensures that certain genes will keep silent before the cell density of the species pass a threshold.

Prisoners’ Dilemma----It is the dilemma in which the two suspects could either choose to cooperate with or betray each other. In conditions when they could communicate freely with each other, they will cooperate, which maximizes their benefits as a whole; while when they are inquisited separately, they will both choose to betray one another to lower the risks of long sentence.

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