IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration
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(New page: =Method= *An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) *Dilutions were plated out at 10<sup>-5</s...) |
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*An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) | *An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) | ||
*Dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions. | *Dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions. | ||
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[[Image:0D600_curve.JPG | 800px]] | [[Image:0D600_curve.JPG | 800px]] | ||
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Latest revision as of 20:04, 29 October 2008
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OD600Calibration Method
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