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Purdue/18 July 2008
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| + | mCherry bacteria are STILL not red. Looked up on Clontech (website selling mCherry)--found that maybe antibodies are necessary to visualize? | ||
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| + | I'm thinking it was a bad assumption to make that mCherry would make bacteria red in visible light just like mRFP-1. | ||
| + | I emailed Katie Clifford my problem. | ||
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| + | From several books, it has been documented that LacZ takes around 2-5 minutes to be transcribed into beta-galactosidase. Still need to find time it takes to diffuse from the cell and cleave X-gal. Perhaps is negligible? Miller (1972) details an experiment about timing gene expression using beta-gal. | ||
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| + | '''Edited by Janie Stine''' | ||
Latest revision as of 18:11, 18 July 2008
Click Here to return to the notebook.
mCherry bacteria are STILL not red. Looked up on Clontech (website selling mCherry)--found that maybe antibodies are necessary to visualize?
I'm thinking it was a bad assumption to make that mCherry would make bacteria red in visible light just like mRFP-1. I emailed Katie Clifford my problem.
From several books, it has been documented that LacZ takes around 2-5 minutes to be transcribed into beta-galactosidase. Still need to find time it takes to diffuse from the cell and cleave X-gal. Perhaps is negligible? Miller (1972) details an experiment about timing gene expression using beta-gal.
Edited by Janie Stine
